Practical
Practical classes will develop skills including:
The manipulation and handling of recombinant organisms and molecules with emphasis to CGLP and health and safety, monitoring, recording and analysing experimental data in molecular biology, operating the range of instrumentation specified safely and effectively in the laboratory, effective group work and good written and oral communication skills, safety in the molecular biology lab, specific hazards and risks, waste disposal of EtBr, UV visualisation, DNA isolation, DNA quantification and visualisation, gel electrophoresis, restriction enzyme digestion of DNA and cloning, transformation of cells with recombinant DNA molecules, DNA amplification using the polymerase chain reaction, demonstration of the effects of heat and pH on DNA, detection of endotoxins, the LAL test, production of pyrogen-free water, basic serological techniques, agglutination reactions, ELISA testing. The application of bioinformatics to discover variability in sequences and trace the effects of molecular evolution in related genes and proteins.
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Medical Microbiology & Immunology:
An introduction to medical microbiology; microorganisms as agents of disease. The human body as a microbial environment. The resident flora and non-specific defence mechanisms.
Endooxins and exotoxins. The importance of endotoxins in the pharmaceutical industry. Structure of the Gram Negative cell wall. LAL testing. Monitoring of the factory environment.
The immune system, including humoral and cell-mediated immunity. The structure of antibodies. Antibody and antigen reactions. Immediate and delayed hypersensitivity. The use of antibodies as diagnostic agents. Monoclonal antibodies. Agglutination tests. ELISA testing.
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